| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Weitzeil, Abbigail R (19)
|
CACANY |
24.86 |
25.00 |
0.14 |
| 2 |
Arcila, Isabella (21)
|
NTSMU |
25.62 |
25.94 |
0.32 |
| 3 |
Gates, Melissa C (25)
|
CATROJ |
25.68 |
26.04 |
0.36 |
| 4 |
King, Shayla O (23)
|
NMUNM |
26.57 |
26.20 |
-0.37 |
| 5 |
Nevalainen, Lotta (21)
|
NCNCS |
25.80 |
26.31 |
0.51 |
| 6 |
Ginnis, Morgan L (19)
|
NMUNM |
26.37 |
26.39 |
0.02 |
| 7 |
Rogers, Hannah L (21)
|
NTSMU |
26.16 |
26.40 |
0.24 |
| 8 |
Koens, Jovanna (20)
|
AZGCU |
26.47 |
26.42 |
-0.05 |
| 9 |
Filova, Katarina (26)
|
FLPSA |
25.90 |
26.49 |
0.59 |
| 10 |
Cunningham, Victoria E (21)
|
NTSMU |
26.09 |
26.51 |
0.42 |
| 11 |
Bonnema, Erika W (21)
|
NCNCS |
25.00 |
26.55 |
1.55 |
| 12 |
Hatayama, Leah A (21)
|
CCFRES |
26.36 |
26.61 |
0.25 |
| 13 |
Center, Victoria A (16)
|
WIBAC |
26.29 |
26.62 |
0.33 |
| 14 |
Ballenger, Marie A (29)
|
PCTOC |
26.64 |
26.67 |
0.03 |
| 15 |
Heyliger, Rebecca M (23)
|
CATROJ |
26.49 |
26.75 |
0.26 |
| 16 |
Knueppel, Jade S (15)
|
WISSTY |
26.50 |
26.80 |
0.30 |
| 17 |
Simmons, Caroline I (24)
|
FGPAQ |
27.03 |
26.99 |
-0.04 |
| 18 |
Ramey, Kenna N (19)
|
PNKING |
26.24 |
27.03 |
0.79 |
| 19 |
Donley, Miranda E (16)
|
OHOSSC |
26.24 |
27.08 |
0.84 |
| 20 |
Vorrasi, Catherine M (32)
|
AZUN-0 |
26.60 |
27.24 |
0.64 |
| 21 |
Braun, Erika L (43)
|
NCMOR |
26.53 |
27.50 |
0.97 |
| 22 |
Ashmore, Jordan W (16)
|
ORTDPS |
27.09 |
27.51 |
0.42 |
| 23 |
O'Brien, Ashleen S (15)
|
PCOAPB |
27.70 |
28.48 |
0.78 |